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Image Search Results
Journal: bioRxiv
Article Title: Human SHED-derived extracellular cues activate a specialized neuroprotective and regenerative program in developing retinal ganglion cells
doi: 10.64898/2026.06.25.733625
Figure Lengend Snippet: (a) Immunofluorescence staining showing expression of Vimentin (green) and CD105 (red) in DPSC and SHED compared with HaCaT epithelial controls. (b) STRO-1 (green) immunostaining in SHED. Nuclei were counterstained with DAPI (blue). Scale bar = 100μm (c) Representative flow cytometry analyses demonstrating expression of mesenchymal stem cell markers (CD73, CD90, CD105, HLA-ABC) and absence of hematopoietic markers (CD34, CD45). All experiments were perfomed at passage 5 (d) Growth curves comparing cumulative population doublings of DPSC and SHED derived from four independent donors across serial passages. SHED displayed enhanced proliferative capacity and reduced donor-to-donor variability.
Article Snippet: Antibodies against the following human antigens were used:
Techniques: Immunofluorescence, Staining, Expressing, Immunostaining, Flow Cytometry, Derivative Assay
Journal: Journal of cellular and molecular medicine
Article Title: Insights From Amniotic and Umbilical Cord Mesenchymal Stem Cells in Wound Healing.
doi: 10.1111/jcmm.70679
Figure Lengend Snippet: FIGURE 1 | Isolation and characterisation of UC-MSCs and A-MSCs. (A) Both UC-MSCs and A-MSCs exhibit a typical spindle-shaped morphol- ogy. Scale bar = 100 μm. (B) The proliferation of the two cells was evaluated by CCK8 assay. (C) These cells are positively stained for the common MSC-associated markers, CD73, CD90, CD105, and have no expression of haematopoietic markers CD45, CD34 and HLA-DR. (D) Following the cul- ture in a specific differentiation medium for 2–3 weeks, the isolated UC-MSCs and A-MSCs successfully differentiated into mesenchymal derivatives, including osteoblasts (Alizarin Red labeling), adipocytes (Oil Red O labeling) and chondrocytes (Alcian blue). Scale bar = 50 μm. (E) The transwell was adopted to evaluate the migration ability of the two types of cells. Scale bar = 50 μm. (F) The wound healing assays were further adopted for the migration ability of the cells. Scale bar = 100 μm.
Article Snippet: Additionally, phenotypic characteristics of UC- MSCs and A- MSCs at passage 3 were analysed by flow cytometry using antibodies against CD14 (BD Biosciences; 555397), CD34 (BD Biosciences; 555822), CD73 (BioLegend; 344004),
Techniques: Isolation, CCK-8 Assay, Staining, Expressing, Labeling, Migration
Journal: Experimental and Therapeutic Medicine
Article Title: Xenotransplantation of human dental pulp stem cells in platelet-rich plasma for the treatment of full-thickness articular cartilage defects in a rabbit model
doi: 10.3892/etm.2019.7499
Figure Lengend Snippet: List of antibodies used for flow cytometry.
Article Snippet:
Techniques: Cytometry